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Neutrophil accumulation after contraction‐induced muscle injury is dependent upon P‐ and E‐selectin (1102.13)
Author(s) -
Sloboda Darcee,
Brooks Susan
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.1102.13
Subject(s) - contraction (grammar) , infiltration (hvac) , chemistry , skeletal muscle , inflammation , muscle contraction , antibody , medicine , immunology , materials science , composite material
Neutrophils accumulate in skeletal muscle after contraction‐induced injury and are thought to exacerbate damage to muscle fibers. The mechanism by which neutrophils enter the muscle is not known, but adhesion proteins on vascular endothelial cells may play a role. We tested the hypothesis that the adhesion proteins P‐ and E‐selectin are critical for neutrophil accumulation after contraction‐induced injury and that blunting neutrophil accumulation would reduce damage. To test our hypothesis, we injured mouse muscles in situ with a series of lengthening contractions. Mice were injected with either antibodies that block P‐ and E‐selectin or control antibodies. In control muscles, injury caused a 50% force deficit, overt damage to muscle fibers and a 15‐fold increase in neutrophil content after two days. Mice treated with blocking antibodies to P‐ and E‐selectin showed a decrease in neutrophil content but no change in force deficit or damage to muscle fibers. Our data suggests that neutrophil accumulation after contraction‐induced injury is dependent upon P‐ and E‐selectin but that blunting neutrophil infiltration after contraction‐induced injury does not always reduce damage. Grant Funding Source : AG000114