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Decreased plasma CO 2 induces redistribution of the V‐ATPase in the choroid plexus epithelium: involvement in regulation of cerebrospinal fluid pH? (1098.4)
Author(s) -
Christensen Henriette,
Damkier Helle,
Paunescu Teodor,
Brown Dennis,
Praetorius Jeppe
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.1098.4
Subject(s) - choroid plexus , extracellular , cerebrospinal fluid , v atpase , atpase , cytoplasm , intracellular , microbiology and biotechnology , biology , chemistry , biochemistry , endocrinology , enzyme , central nervous system , neuroscience
Little is known about the mechanisms behind cerebrospinal fluid (CSF) pH control. The CSF is produced by the choroid plexus epithelium (CPE), located in the ventricular system of the brain. It is believed that CSF pH is regulated by the CPE but the mechanism is not yet understood.The CPE expresses several acid/base transporting proteins. One of these is the V‐ATPase, which is found in nearly all cell types where it acidifies intracellular organelles. However, in some cell types the protein acidifies the extracellular milieu upon translocation to the plasma membrane. In this study we have investigated the subcellular localization of the V‐ATPase in the CPE at normal and low levels of pCO 2 . Mice were subjected to acute hyperthermia, causing hyperventilation and a decrease in blood pCO 2 . Immunohistochemical analysis of the CPE was carried out on the paraffin embedded brain sections by fluorescence‐ and electron microscopy using an antibody against the A subunit of the V‐ATPase.Under control conditions, the V‐ATPase was located in numerous vesicles that were evenly distributed throughout the cytoplasm. However, during hyperthermia, a statistically significant redistribution of the V‐ATPase towards both the luminal and basolateral plasma membrane domains of CPE cells was found. This indicates that the V‐ATPase in the CPE responds to systemic acid/base conditions and may help guard the CSF against large changes in pH.