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Effect of altered endogenous melatonin concentration by conditional light control on cellular organization in injured spinal cord (1096.2)
Author(s) -
Jin Yunho,
Hong Yunkyung,
Park Kanghui,
Hong Yonggeun
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.1096.2
Subject(s) - melatonin , neun , spinal cord , endogeny , cerebrospinal fluid , spinal cord injury , medicine , endocrinology , anesthesia , immunohistochemistry , psychiatry
Spinal cord injury(SCI) causes primary and secondary damage, leading to neurologic deficits. We aimed to determine the effect of altered melatonin level in cerebrospinal fluid(CSF) by conditional light control on cellular organization and motor recovery of SCI rats. Rats were divided into 3 groups and pre‐adapted under each condition for at least 2 weeks prior to operation; L/D(12/12‐hr light/dark), LL(24‐hr constant light), DD(24‐hr constant dark). After microsurgery, we collected injured cord segments and CSF. Rats under DD condition exerted higher BBB scores than that of L/D from day 7 through 28, while LL condition induced reduction of BBB scores than L/D on day 21 and 28. Melatonin concentration in CSF was the highest in DD on day 3, which was concomitant with NeuN upregulation in injured cord lesions. Elevated melatonin concentration in DD condition was sustained day 7 only. Although NeuN expression was significantly increased in all of groups at day 28 compared to day 3, its expression was the lowest in LL condition. Olig2 was also raised in DD condition on day 28. These results suggest that higher endogenous melatonin concentration in CSF may prevents neuronal cell loss but also promotes oligodendrocyte differentiation in injured cord region, leading to motor recovery in SCI rats. Key words: Spinal cord injury, Cerebrospinal fluid, Endogenous melatonin, Light control, NeuN