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Increases in protein expression of the alpha7beta1 integrin pathway following 8 weeks of acetaminophen administration in the rat soleus (1095.17)
Author(s) -
Gallagher Philip,
Graham Zachary,
Broderick Tom,
Carroll Chad
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.1095.17
Subject(s) - sed , soleus muscle , medicine , endocrinology , phosphorylation , acetaminophen , chemistry , pharmacology , skeletal muscle , biochemistry
Acetaminophen (APAP) has been shown to increase muscle mass following chronic exercise. Proteins of the alpha7beta1 integrin complex can detect stretch along the sarcolemma and activate signaling pathways that are responsible for cytoskeletal rearrangement and muscle hypertrophy. The purpose of this study was to determine if 8 weeks of aerobic exercise with or without APAP would effect expression of alpha7beta1 integrin, the FAK/cSrc/ERK1/2 cascade and certain hypertrophic proteins of the PI3K pathway in rat soleus muscle. Breifly, 10 week‐old male Wistar rats were randomly assigned to either a sedentary+placebo group (SED+PLAC; n=6), a sedentary+APAP group (SED+APAP; n=6), an exercise+placebo group (EX+PLAC; n=6) or an exercise+APAP (EX+APAP; n=6). Rats ran in progressive volumes (up to 60min/day) at 20m/min with an 8° grade for 8 weeks. Rats in the APAP groups received 200mg/kg/day of APAP via oral gavage. Protein expression was measured using Western immunoblotting. We found that rats that were administered APAP had higher levels of the 70 kDa alpha7 integrin subunit, total FAK, cSrc and p70s6k independent of exercise. Rats in the SED+APAP group had higher expression of total 4eBP1 compared to all other groups and rats in the EX+APAP group has higher expression of phosphorylated cSrc(416) compared to all other groups. Levels of phosphorylated 4eBP1(37/46) in the SED+APAP were higher compared to SED+PLAC. Finally, there were no differences between any group in the levels of expression for the full length alpha7 subunit, the beta1 integrin subunit, alpha‐actinin, total and phosphorylated ERK1/2(202/204), phosphorylated FAK(397) and phosphorylated p70s6k(389). In conclusion, consumption of APAP is associated with large changes in expression of multiple proteins of the focal adhesion independent of aerobic exercise in the rat soleus. Furthermore, APAP consumption is related to increases in expression of proteins of the hypertrophic PI3K pathway.