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Inhibitory effects of J10 on lung cancer cell motility: an in vitro and in vivo study (1095.14)
Author(s) -
Wu ChiehHsi,
Chen ShuoChueh,
Chien YiChung,
Pan ChunHsu,
Chen ChihYi,
Sheu JyhHorng
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.1095.14
Subject(s) - lung cancer , metastasis , motility , in vivo , protein kinase b , cancer , cancer research , pi3k/akt/mtor pathway , western blot , chemistry , zymography , matrix metalloproteinase , phosphorylation , medicine , apoptosis , biology , biochemistry , microbiology and biotechnology , gene
There are many major causes of cancer death, including metastasis of cancer. Dihydroaustrasulfone alcohol, which isisolated from marine coral, has shown antioxidant activity, but has not been reported to have an anti‐cancer effect. We first discovered that dihydroaustrasulfone alcohol provided a concentration‐dependent inhibitory effect on the migration and motility of human non‐small cell lung carcinoma (NSCLC) A549 cells by trans‐well and wound healing assays. The results of a zymography assay and Western blot showed that dihydroaustrasulfone alcohol suppressed the activities and protein expression of matrix metalloproteinase (MMP)‐2 and MMP‐9. Further investigation revealed that dihydroaustrasulfone alcohol suppressed the phosphorylation of ERK1/2, p38, and JNK1/2. Dihydroaustrasulfone alcohol also suppressed the expression of PI3K and the phosphorylation of Akt. Furthermore, dihydroaustrasulfone alcohol markedly inhibited tumor growth in Lewis lung cancer (LLC)‐bearing mice. We concluded that Dihydroaustrasulfone alcohol is a new pure compound with anti‐migration and anti‐tumor growth activity in lung cancer and might be applied to clinical treatment in the future. Grant Funding Source : Supported by National Science Council, Taiwan with the Grant No: NSC 101‐2320‐B‐039 ‐058 a