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Role of CFTR in embryonic stem cell programming (1094.4)
Author(s) -
Liu Zhenqing,
Yuan Ping,
Jiang Xiaohua,
Chan Hsiao Chang
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.1094.4
Subject(s) - wnt signaling pathway , microbiology and biotechnology , embryonic stem cell , homeobox protein nanog , sox2 , biology , cystic fibrosis , stem cell , cellular differentiation , cyclin e1 , cystic fibrosis transmembrane conductance regulator , downregulation and upregulation , cell , cell cycle , genetics , induced pluripotent stem cell , signal transduction , gene , cyclin d1
Embryonicstem cells (ESC) are unique cell types characterized by their capacity of self‐renewal and potential of differentiation. The balance between self‐renewal and differentiation in ESC is controlled by a complex interplay.Cystic fibrosis transmembrane conductanceregulator (CFTR) is a cAMP‐regulated anion channel which is predominantly expressed in a wide range of epithelial tissues. Mutations in the gene encoding CFTR cause cystic fibrosis. Recently, we have found that CFTR is functionally expressed in both human and mouse ESC. To further understand CFTR function in ESC pluripotency and differentiation, we derived CFTR mutated (DF508) ESC with C57BL6 background. We found that DF508 ESC proliferate faster than wild type cells, and had increased expression of self‐renewal markers such as Oct4, Nanog and Sox2. Wnt/β‐catenin pathway is an important pathway that controls mouse ESC pluripotency. Intriguingly, our western Blot analysis revealed that Cyclin D2, a downstream target gene of β‐catenin, was significantly upregulated in DF508 ES cells. Taken together, our results suggest CFTR may play a role in regulation of ESC pluripotency and differentiation.