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Chronic placental ischemia alters expression of placental and fetal insulin‐like growth factor proteins in the rat (1085.12)
Author(s) -
Beasley Kara,
Needham Karen,
Regal Jean,
Lovering Andrew,
Gilbert Jeffrey
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.1085.12
Subject(s) - bronchopulmonary dysplasia , fetus , medicine , preeclampsia , intrauterine growth restriction , endocrinology , placenta , ischemia , gestation , placental growth factor , birth weight , insulin like growth factor , offspring , pregnancy , growth factor , gestational age , vascular endothelial growth factor , biology , receptor , genetics , vegf receptors
Preeclampsia is often characterized by placental ischemia and dysregulation of angiogenic growth factors such as IGF and is a major cause of low birth weight and preterm deliveries. These offspring are at increased risk for chronic diseases such as bronchopulmonary dysplasia (BPD), a hypoplastic lung disease defined by preterm birth and neonatal O2 therapy. While O2 therapy and preterm birth clinically define BPD, current models of BPD employ hyperoxia in normally grown term neonates without considering the role of low birth weight and altered fetal endocrine milieu. To that end, we hypothesized chronic placental ischemia would decrease expression of insulin like growth factors (IGF) I and II and related proteins in placental and fetal tissues. Chronic placental ischemia was achieved using the reduced uterine perfusion pressure (RUPP; n 蠅7) model of preeclampsia and fetal growth restriction. Normal pregnant (NP; n 蠅8) rats served as controls and tissues were collected on day 19 of gestation. Placental IGF‐I was decreased in RUPP compared to NP (89.8 ± 10.9 vs. 133.0 ± 13.1ng/ml, p<0.05). Conversely, amniotic fluid IGF‐I (0.25 ± 0.04 vs. 0.16 ± 0.02pg/ml, p<0.05) and IGF‐II (62.7 ± 8.2 vs. 42.0 ± 5.5 pg/ml, p<0.05) were increased in RUPP vs. NP. Fetal hepatic IGF‐2 was increased in RUPP (155.6 ± 12.6 vs. 117.0 ± 6.3pg/ml, p<0.05). Finally, fetal lung IGF‐IRα was not different between the groups. These data indicate chronic placental ischemia alters expression of IGF proteins in placental and fetal tissues and could alter the developmental trajectory of programming susceptible organs such as the lung and kidney. Grant Funding Source : Supported by NIH RO1HL114096 and AHA 10SDG2600040