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A reduction in uterine perfusion pressure induces hypertension during pregnancy in the mouse (1084.5)
Author(s) -
Intapad Suttira,
Warrington Junie,
Spradley Frank,
Palei Ana,
Drummond Heather,
Ryan Michael,
Granger Joey,
Alexander Barbara
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.1084.5
Subject(s) - perfusion , medicine , preeclampsia , blood pressure , fetus , intrauterine growth restriction , mean arterial pressure , pregnancy , endocrinology , gestation , abdominal aorta , aorta , cardiology , biology , heart rate , genetics
Preeclampsia is a hypertensive disorder of pregnancy and a leading cause of maternal morbidity and mortality; however, the underlying mechanisms are not clear. Reduced uterine perfusion in the pregnant rat induces many of the characteristics of preeclampsia including hypertension and fetal growth restriction. However, the lack of availability of a mouse model prevents harnessing the power of genetically modified mice. Thus, the aim of this study was to develop a mouse model of reduced uterine perfusion pressure (RUPP). Pregnant C57BL/6J mice were subjected to either a sham surgery or a mechanical reduction of the blood flow to the abdominal aorta and both branches of ovarian arteries initiated at day 13 of gestation (E13). Mice were instrumented with a carotid arterial catheter at E17 followed by measurement of mean arterial pressure (MAP) at E18 in the conscious state. MAP at E18 in the RUPP was significantly elevated versus sham (117±4 vs. 97±2 mmHg, P <0.01; respectively). Pup weight from the RUPP were significantly reduced versus sham (0.77±0.02 vs. 0.92±0.03 g, P <0.003; respectively). However, no significant difference in placental weight was noted between RUPP and sham mice (0.07±0.003 vs. 0.08±0.004 g; respectively). Thus, these data indicate that RUPP induces hypertension and fetal growth restriction in the mouse. Importantly, this mouse model of RUPP will be a valuable tool to address novel hypotheses related to the etiology of preeclampsia.