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Thermal stress conditions endothelial cells and improves cellular function (1084.10)
Author(s) -
Needham Karen,
Macrorie Sarah,
Minson Christopher,
Gilbert Jeffrey
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.1084.10
Subject(s) - heat shock protein , andrology , heat shock , angiogenesis , chemistry , umbilical vein , microbiology and biotechnology , medicine , endocrinology , immunology , biology , in vitro , biochemistry , gene
It is well established that temporary exposure to a stressor, such as heat, can induce the production of proteins that confer cellular protection (heat shock proteins ‐ HSP). Our lab has shown that exercise training (a stressor) in pregnant rats increases production of HSP 27, 60, and 90 in the placenta. The aim of this study is to determine whether heat shock, resulting in the up‐regulation of HSPs, improves endothelial function in human umbilical vascular endothelium (HUVEC) treated with normal pregnant rat serum or serum from rats with placental ischemia induced hypertension (RUPP). HUVECs were heat shocked at 42.5° C for 4hrs followed by 2hr recovery at 37° C. Extracts from shocked cells had 4x HSP 70 (p<0.01) and 3x HSP 27 (p<0.01) protein levels over the control. Shocked cells and 37° C control cells were treated with NP or RUPP serum and function was assessed with angiogenesis and wound healing assays. Tubule length counts in heat treated cells were at three times the level of no shock controls (p <0.01), without any effect from serum. In the wound assay RUPP/heat shocked cells tended toward greater mitogenesis and motility. These benefits may involve endothelin 1, which has been shown to promote HUVEC proliferation and migration and was elevated by 20% (p<0.05) in the media of RUPP/heat shock HUVECS in our study. These results suggest that human endothelial cells may improve function with conditioning by low level stressors. Such conditioning, as would be conferred by exercise, may improve the outcome for women with preeclampsia. Grant Funding Source : Supported by NIH RO1HL114096 and AHA 10SDG2600040