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Female spontaneously hypertensive rats have a compensatory increase in renal regulatory T cells in response to elevated blood pressure (1083.3)
Author(s) -
Tipton Ashlee,
Li Beverly,
Sullivan Jennifer
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.1083.3
Subject(s) - hydrochlorothiazide , medicine , endocrinology , blood pressure , infiltration (hvac) , flow cytometry , kidney , immunology , physics , thermodynamics
We recently demonstrated that female SHR have more anti‐inflammatory regulatory T cells (Tregs) in their kidneys than males. The goal of this study was to determine the impact of blood pressure (BP) on Tregs in male (M) and female (F) SHR. We hypothesized that hypertension (HTN) promotes renal T cell infiltration in both sexes, although females will have greater increases in Tregs. SHR were treated with hydrochlorothiazide and reserpine from 6 to 13 wks of age (7‐HCTZ), 11 to 13 wks of age (2‐HCTZ), or vehicle control (N=5‐8) and renal T cells were measured by flow cytometry. 7‐HCTZ attenuated age‐related increases in BP (M: 137±4, F: 132±4 systolic BP mmHg) and 2‐HCTZ reversed established HTN (M: 122±4, F: 116±3 mean arterial BP mmHg; p<0.05) compared to vehicle‐treated SHR (M: 174±4, F: 161±1 mean arterial BP mmHg). Neither 7‐HCTZ nor 2‐HCTZ altered renal CD3 + or CD4 + T cell infiltration in either sex. Both treatments decreased renal Tregs in female SHR (vehicle: 11±0.2 vs 7‐HCTZ: 6±0.4 %CD4 + CD3 + cells, p<0.05; vehicle: 9±0.5 vs 2‐HCTZ: 3±0.6, p<0.05); Tregs in males were unaffected. F SHR maintained greater Treg infiltration than males following 7‐HCTZ treatment (males: 7±1 vs females: 6.4±0.4; p<0.05). Treg infiltration was comparable in 2‐HCTZ‐treated M and F SHR (Tregs males: 5±1 vs females: 3.2±0.6, NS). These data suggest that female SHR have a compensatory increase in renal Tregs in response to increases in BP. Grant Funding Source : Supported by Intramural Funds