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Inhibition of endoplasmic reticulum stress does not alter blood pressure in adult male and female spontaneously hypertensive rats (1083.2)
Author(s) -
Crislip Gene,
Williams Eric,
Tipton Ashlee,
Sullivan Jennifer
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.1083.2
Subject(s) - endocrinology , unfolded protein response , blood pressure , medicine , chop , endoplasmic reticulum , basal (medicine) , adult male , western blot , chemistry , chemotherapy , biochemistry , insulin , gene
ER stress has recently been linked to the development of hypertension in male SHR. Little is known regarding ER stress in females, although blood pressure (BP) in female SHR is lower than in age‐matched males. This study was designed to test the hypothesis that female SHR have less ER stress than males, and consequently will have a smaller decrease in BP in response to an ER stress inhibitor. 11‐week old rats were treated with vehicle or the ER stress inhibitor phenylbutyric acid (PBA) for 2 weeks (100 mg/kg/day via drinking water; N=4) and BP was measured weekly via tail cuff. The ER stress markers glucose‐regulated protein (GRP78) and C/EBP homologous protein (CHOP) were measured in the renal cortex via Western blot analysis. Basal expression of CHOP was higher in females when compared to males (female: 2.7±0.4 vs. male: 1.3±0.2, P=0.007) as well as with GRP (female: 1.8±0.2 vs. male: 1.3±0.1, P=0.04), however these differences were abolished after treatment. BP did not change in either sex following PBA treatment (female: 169±8 to 174±6 vs. male: 182±4 to 183±5 mmHg, NS). In conclusion, regulation of blood pressure in adult SHR is not mediated by ER stress. Future studies will determine if inhibition of ER stress prior to the development in hypertension effects BP.