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Adaptation to chronic hypoxia improves cardiac ischemic tolerance in spontaneously hypertensive rats (1080.3)
Author(s) -
Neckar Jan,
Brabcova Iveta,
Weissova Romana,
Zajickova Pavlina,
Mandikova Petra,
Silhavy Jan,
Zurmanova Jitka,
Novakova Olga,
Kalous Martin,
Pravenec Michal,
Kolar Frantisek
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.1080.3
Subject(s) - medicine , hypoxia (environmental) , endocrinology , superoxide dismutase , ischemia , thioredoxin , catalase , cytosol , messenger rna , chemistry , oxidative stress , enzyme , gene , biochemistry , oxygen , organic chemistry
The effect of adaptation to chronic continuous hypoxia (CCH) on cardiac ischemic tolerance and the expression of selected mitochondrial proteins, antioxidative enzymes and signalling molecules were analysed in left ventricles of spontaneously hypertensive rats (SHR) and in a novel conplastic strain SHR‐mt BN . These animals are characterized by a selective replacement of the mitochondrial genome of SHR with that of Brown Norway strain, which is more tolerant to ischemic injury. Rats were kept 21 days at CCH (inspired O 2 fraction 0.1). CCH reduced the infarct size induced by coronary occlusion from 71±4% of the area at risk in normoxic SHR to 49±6 % in hypoxic SHR. It was accompanied by decreased protein levels of complex IV, cytosolic superoxide dismutase and mRNA levels of thioredoxin enzymes. On the other hand, mRNA levels of catalase, hexokinase 2 (HK2) and hypoxia‐inducible factor 1α (HIF‐1α) were increased in hypoxic SHR. As compared to SHR, CCH induced more pronounced infarct size‐limiting effect (by 19 %), elevated HK2 and HIF‐1α mRNA levels (by 36 and 25 %) and reduced complex IV proteins (by 13 %) in SHR‐mt BN . Overall, we demonstrate that CCH protects SHR hearts against acute ischemia/reperfusion injury and that mitochondrial genes can contribute to this protective effect.