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Caffeine increases basal eNOS phosphorylation and stimulates endothelial cell growth (1075.6)
Author(s) -
Calleo Adrianna,
Harris M. Brennan
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.1075.6
Subject(s) - enos , caffeine , umbilical vein , phosphorylation , endothelial stem cell , cell growth , endocrinology , human umbilical vein endothelial cell , medicine , chemistry , biology , nitric oxide , biochemistry , in vitro , nitric oxide synthase
Caffeine is one of the most widely used neural stimulants and has both vasodilator and vasoconstrictor effects. In this study, we examined the effects of caffeine on eNOS phosphorylation and on endothelial cell growth. eNOS phosphorylation was examined in human umbilical vein cells (HUVECs) in the absence and presence of the eNOS agonist VEGF using Western blot. Endothelial cell growth was determined using a scratch wound assay in HUVECs. eNOS phosphorylation was increased in HUVECs treated with 100 µM caffeine and 1 mM caffeine for 1 h or 20 ng/ml VEGF for 30 min. No further increases in phosphorylation were observed in cells treated with both caffeine and VEGF. Endothelial cell growth was increased in cells exposed to 100 µM and 1 mM caffeine. Higher concentrations of caffeine impeded cell growth. In conclusion, these data suggest that caffeine acts as an eNOS agonist during short exposures which can augment endothelial cell growth. Grant Funding Source : Supported by NIH/NHLBI 1R15HL108428