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Study on penile corpus cavernosum relaxation induced by Berberis amurensis Rupr (1075.10)
Author(s) -
Tan Rui,
Kim Hye Yoom,
Cho Kyung Woo,
Kang Dae Gill,
Lee Ho Sub
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.1075.10
Subject(s) - iberiotoxin , apamin , charybdotoxin , tetraethylammonium , chemistry , nitric oxide , enos , endocrinology , channel blocker , berberis , phenylephrine , muscle relaxation , papaverine , medicine , potassium channel , nitric oxide synthase , traditional medicine , potassium , blood pressure , calcium , organic chemistry
Study on Penile corpus cavernosum relaxation induced by Berberis amurensis Rupr Rui Tan 1, 2, , Hye Yoom Kim 1,2 , Kyung Woo Cho 2 , Dae Gill Kang 1,2 , and Ho Sub Lee 1,2 * 1 College of Oriental Medicine and Professional Graduate School of Oriental Medicine, Wonkwang University, Iksan, Jeonbuk 570‐749, Republic of Korea; 2 Hanbang Body‐fluid Research Center, Wonkwang University, Shinyong‐dong, Iksan, Jeonbuk, 570‐749, Republic of Korea The aim of the present study is to investigate whether a methanol extract of Berberis amurensis Rupr (BAR) augments penile erection. Preconstracted with phenylephrine (PE) in isolated endothelium‐intact rabbit corpus cavernosum, BAR relaxed penile smooth muscle in a dose‐dependent manner, which was inhibited by pretreatment with N G ‐nitro‐L‐arginine methyl ester (L‐NAME), a nitric oxide synthase inhibitor, and 1H‐[1,2,4]‐oxadiazole‐[4,3‐α]‐quinoxalin‐1‐one (ODQ), a soluble guanylyl cyclase (sGC) inhibitor. BAR‐induced relaxation was significantly attenuated by pretreatment with tetraethylammonium (TEA), a nonselective K + channel blocker for BK Ca, IK Ca and SK Ca , and charybdotoxin, iberiotoxin, apamin, a selective Ca 2+ sensitive K + channel inhibitor, respectively. BAR increased cGMP levels of the corpus cavernosum in a concentration‐dependent manner. In addition, BAR caused increase of peak intracavernous pressure (ICP), ICP/MAP ratio and area under the carve (AUC) in a dose dependent manner in SD rats. Taken together, these results suggest that BAR augments penile erection via NO/cGMP system and Ca 2+ sensitive‐K + Channel in corpus cavernosum.