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A potential role of memory T cells in hypertension (1074.1)
Author(s) -
Itani Hana,
Zhang Feng,
Xiao Liang,
Chen Wei,
Harrison David
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.1074.1
Subject(s) - cd8 , flow cytometry , spleen , immune system , effector , kidney , cytotoxic t cell , blood pressure , immunology , acquired immune system , angiotensin ii , renin–angiotensin system , chemistry , medicine , endocrinology , biology , in vitro , biochemistry
Immunological memory is an important component of adaptive immunity. We have previously shown that T cells are important in hypertension. We therefore hypothesized that memory T cells contribute to long‐term and repeated hypertensive challenges. To test this, mice received 2 weeks of angiotensin II, were allowed to recover for 3 weeks and then were received a low dose of ang II (140 ng/kg/min) that minimally raises blood pressure in naïve mice. This low‐dose of ang II increased blood pressure to 137±6 mmHg in mice that had previously received a vehicle infusion, but to 172±12 mmHg in mice that had received ang II. In keeping with a memory T cell response, we found that ang II increased CD8+ central memory cells in the kidney 5 to 10‐fold while reducing these effector memory CD8+ T cells in the spleen. CD8+ memory T cells require co‐stimulatory signaling between CD70 on macrophages and CD27 on T cells. We found that ang II infusion increased CD70 mRNA in isolated kidney vessels by 5 to 10‐fold. Similarly, flow cytometry revealed that ang II increased macrophages expressing CD70 and CD8+ T cells expressing CD27 expression in the kidney. Thus, these studies indicate that repeated exposures to ang II promote an immune memory response of CD8+ T cells and markedly enhance the hypertensive response. The role of CD27 and CD70 signaling requires additional study but might serve as a therapeutic target.