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Mapping the molecular dynamics of clathrin‐mediated endocytosis (107.3)
Author(s) -
Merrifield Christien
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.107.3
Subject(s) - endocytosis , endocytic cycle , clathrin , receptor mediated endocytosis , microbiology and biotechnology , cytoskeleton , receptor , vesicle , chemistry , cell membrane , fluorescence microscope , cell , biophysics , membrane , biology , biochemistry , fluorescence , physics , quantum mechanics
Clathrin mediated endocytosis is the principle mechanism by which cells internalize cell surface receptors. It can be thought of as a molecular machine which concentrates receptors into patches at the plasma membrane and which bends the membrane into a vesicle that pinches off. Quite how the many structural, enzymatic and cytoskeletal components of the endocytic machine are spatially and temporally organised is not fully understood. In this talk I will describe our efforts to address this problem by analysing single endocytic events using total internal fluorescence microscopy (TIR‐FM).