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Regulation of phenotype‐associated phosphodiesterase expression in human vascular smooth muscle cells by co‐culture with endothelial cells: role of shear stress (1065.6)
Author(s) -
Hubert Fabien,
Brzezinska Paulina,
Rampersad Sarah,
Maurice Donald
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.1065.6
Subject(s) - vascular smooth muscle , phenotype , microbiology and biotechnology , biology , phosphodiesterase 3 , cell culture , phosphodiesterase , cell , endocrinology , enzyme , biochemistry , smooth muscle , phosphorylation , genetics , gene , protein kinase a
Vascular smooth muscle cells (VSMCs) are differentiated and quiescent (contracile phenotype) in adult blood vessels. In contrast, VSMCs can become activated and highly proliferative and migratory (synthetic phenotype) under certain pathophysiological conditions such as atherosclerosis, or when medial VSMCs are isolated and purified for cell culture. Studies have established that cAMP relaxes contractile VSMCs and inhibits the proliferation and migration of synthetic VSMCs. Furthermore, we previously showed that modification of the expression and activity of phosphodiesterases (PDE), the cyclic nucleotide hydrolysing enzymes, accompanies the phenotypic switch associated with culturing human VSMCs. Vascular endotheliall cells (VECs) can also promote a differentiated VSMC phenotype given their close proximity to VSMCs in vivo. In this context, and focusing on cAMP‐signaling pathways, we are studying the impact of VEC‐VSMC interactions on VSMC phenotypic modulation as well as the effects of shear stress. Our results indicate that VEC‐VSMC co‐culture and laminar shear stress impact phenotype‐associated PDE expression in human VSMCs.