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Early life stress induces bladder dysmotility in adult rats (1065.17)
Author(s) -
Kennedy Dawn,
Mohammadi Ehsan,
Hurst Robert,
GreenwoodVan Meerveld Beverley
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.1065.17
Subject(s) - contractility , detrusor muscle , medicine , carbachol , isometric exercise , endocrinology , stimulation , urinary bladder
Symptoms of painful bladder syndrome (PBS) including urgency and pain are common in female patients who report early life stress (ELS) such as neglect or abuse during childhood. We hypothesized that ELS contributes to PBS by inducing abnormal bladder detrusor muscle contractility in adulthood. This study used an odor‐attachment model in which female rats were conditioned on postnatal days (PND) 8‐12 using predictable or unpredictable odor‐shock conditioning, or an odor only control. In adulthood, bladder muscle strips were harvested and mounted in a muscle bath to measure contractile responses to neurally mediated signalling (electrical field stimulation‐EFS), muscarinic receptor‐dependant signalling with carbachol, and membrane depolarization with KCl. Detruser muscle responses to EFS were significantly decreased (P<0.001) in the unpredictable (12.6±2g/cm 2 ) compared to the predictable (24.9±2g/cm 2 ) or odor only (24.0±1.3g/cm 2 ) groups. Detrusor muscle responses to CCh or KCl were not significantly different between groups. Our data indicate that ELS alters neurally mediated detrusor muscle contractility in the absence of abnormalities in smooth muscle myogenic responses. These findings suggest that therapeutics targeting neural innervation of the bladder musculature could effectively reduce PBS symptoms by normalizing bladder contractility in adulthood. Grant Funding Source : This work was supported by a department of Veterans Affairs Merit Grant to Dr. Beverley Greenwood‐Va