Premium
GABA is an inhibitory neurotransmitter in ganglia of the bivalve mollusc, Crassostrea virginica (1059.4)
Author(s) -
Mathieu Sadchla,
Sylvain Darlene,
Walden Fatima,
Catapane Edward,
Carroll Margaret
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.1059.4
Subject(s) - inhibitory postsynaptic potential , neurotransmitter , serotonergic , excitatory postsynaptic potential , biology , serotonin , dopamine , dopaminergic , medicine , escape response , endocrinology , neuroscience , anatomy , central nervous system , receptor , biochemistry
In most studied bivalves, beating rate of gill lateral cilia is controlled by a reciprocal serotonergic‐dopaminergic innervation from their cerebral ganglion (CG) and visceral ganglia (VG). GABA is a major inhibitory neurotransmitter in molluscs and other animals, but its actions have not been well studied in bivalves. We previously showed GABA inhibited actions of serotonin (HT) on increasing cilia beating when both were applied to the CG of C. virginica. We hypothesize GABA is also an inhibitory neurotransmitter in the VG of C. virginica and inhibits the excitatory HT neurons there as well. We tested this by examining effects of GABA at the VG. Cilia beating rates were measured by stroboscopic microscopy in animal preparations in which innervation of the gill by the CG was cut, but the innervation of the gill by the VG was left intact. Superfusing HT (10 ‐6 ‐ 10 ‐3 M) to the VG produced a dose‐dependent increase in beating from an average basal rates of 10 beats/min to 25 beats/min. Superfusing GABA (10 ‐6 ‐ 10 ‐3 M) did not alter beating. Superfusing HT in the presence of increasing concentrations of GABA resulted in a dose‐dependent blockage of the HT induced response. This study in conjunction with our previous work is showing GABA is working at both the CG and VG as an inhibitory ganglionic neurotransmitter in C. virginica to inhibit the HT neurons that innervate the gill and speed up the beating of the lateral gill cilia. Grant Funding Source : NIH‐2R25GM0600309, NYSED‐0516041071, NSF‐0622197