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In vivo immunomodulatory effect of S22‐Gda (1056.9)
Author(s) -
Jang Yeonjeong,
Kim Jiyun,
Lee Chunggi,
Pyo Suhkneung
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.1056.9
Subject(s) - immune system , macrophage , phagocytosis , in vivo , spleen , cytotoxicity , biology , chemistry , pharmacology , immunology , in vitro , biochemistry , microbiology and biotechnology
S22‐Gda is a purified extract from mixture of Celosia argentea Linne , Allium sativum L. and Plantago asiatica . Many herbal extract mixtures have been known to have various biological activities including stimulation of immune response. However, the biological effects of S22‐Gda have not been addressed. In the present study, we examined immunomodulatory effect of S22‐Gda on peritoneal macrophages and splenic immune cells from six week‐old mice that were fed either a control or oral administration 50 mg/kg of S22‐Gda for 14 days. The results from MTT assay showed that S22‐Gda enhanced macrophages‐ or NK cells‐mediated cytotoxicity to tumor cells. In addition, S22‐Gda significantly increased phagocytosis capacity of peritoneal macrophage. The colorimetric NO assay revealed that NO production was not increased by S22‐Gda in peritoneal macrophage. However, the results from ELISA assay demonstrate that S22‐Gda significantly increased the production of IL‐6, TNF‐α and IFN‐α but not INF‐β in peritoneal macrophage. Overall, our results suggest that S22‐Gda may be a potential immunotherapeutic agent by stimulating macrophages and splenic immune cells functions.