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Evaluation of anti‐inflammatory effect of analogs of L‐carnitine in a model of chronic inflammation in vivo (1056.1)
Author(s) -
MendozaRivera Brissa,
ReyesEsparza Jorge,
De La Cruz Cordero Ricardo,
RodriguezFragoso Lourdes
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.1056.1
Subject(s) - inflammation , granuloma , spleen , splenocyte , in vivo , granuloma formation , epithelioid cell , pathology , infiltration (hvac) , medicine , chemistry , immunology , pharmacology , endocrinology , biology , immunohistochemistry , physics , microbiology and biotechnology , thermodynamics
In this project we developed a model of chronic inflammation induced by subcutaneous implantation of cotton tassel to induce granuloma formation and evaluate drug efficacy of 3 analogs of L‐carnitine. Chronic granulomatous inflammation is a specific type of chronic inflammatory reaction, characterized by the accumulation of modified macrophages (epitheloid cells). Wistar male rats with granuloma were treated with the three analogs at a dose of 2mg/kg. Different serum interleukins were determined and histological analysis of thymus, spleen and granuloma was made. The analysis shows a reduced level of pro‐inflammatory interleukins (Il‐6 and TNF‐α) in animals treated with analogs having a greater effect the analog 2 and 3. Histological analysis showed the presence of epithelioid cells and giant cells in animals treated with L‐carnitine and analog 1, whereas animals treated with analogue 2 and 3 showed leukocyte infiltration. With regard the thymus, only was seen an increase in medulla area with the analog 2. While in the spleen was observed a reduction of medulla area with the analogs 2 and 3. In conclusion the analogs had anti‐inflammatory and anti‐granuloma activity, but they had different efficacy. Grant Funding Source : CONACYT