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Sevoflurane enhances proliferation of human colon cancer HCT116 cells via adenosine triphosphate‐sensitive potassium channels (1052.8)
Author(s) -
Kawaraguchi Yoshitaka,
Sugimoto Hiroshi,
Nomura Yasumitsu,
Furuya Hitoshi,
Kawaguchi Masahiko
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.1052.8
Subject(s) - sevoflurane , cell growth , chemistry , glibenclamide , cancer cell , mtt assay , pharmacology , adenosine triphosphate , potassium channel , cancer , biochemistry , medicine , endocrinology , diabetes mellitus
Introduction: Sevoflurane is one of the commonly used volatile anesthetics in cancer patients. The protective effect of sevoflurane preconditioning against ischemia/reperfusion has raised concerns about whether sevoflurane could acts advantageously for survival even in cancer cells. Therefore, we investigated the effects of sevoflurane on proliferation in cancer cells. Methods: HCT116 cells were plated in 96‐well plates at a density of 1 × 10 4 cells/well and incubated overnight. On the following day, cells were exposed to 1% sevoflurane for 6 h. After 24 h recovery, we performed MTT assay. The absorbance of the formazan product was measured at a wavelength of 570 nm using 650 nm as the reference. In addition, to investigate the role of adenosine triphosphate‐sensitive potassium (K ATP ) channels, we conducted the same experiment under co‐administration with K ATP inhibitor, glibenclamide. Results: Sevoflurane significantly enhanced cell proliferation compared to control (0.53 + 0.023 vs. 0.46+0.033, n=6, p<0.01, respectively). Enhanced proliferation by sevoflurane was completely blocked by co‐administration with glibenclamide. Conclusion: We show that 1% sevoflurane for 6 h enhanced cell proliferation via K ATP channels in HCT116 cells.