Premium
Biomarkers for Western diet accentuated cellular damage in benzo(a)pyrene‐induced colon cancer (1052.2)
Author(s) -
Harris Kelly,
Pulliam Stephanie,
Niaz Mohammad,
Okoro Emmanuel,
Guo Zhongmao,
Washington Mary,
Adunyah Samuel,
Ramesh Aramandla
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.1052.2
Subject(s) - adiponectin , benzo(a)pyrene , medicine , endocrinology , chemistry , dna damage , insulin , carcinogen , biochemistry , insulin resistance , dna
The role of fat content in diet has been linked to the development of several types of cancers. We have investigated the effect of dietary fat type on benzo(a)pyrene (an environmental toxicant)‐induced colon cancer in an adult male rat model, the P olyposis I n the R at C olon (PIRC) kindred type. Groups of PIRC rats (n = 5) were fed with AIN‐76A regular diet (RD) or Western diet (WD) and these rats also received 25,50 and 100 µg B(a)P/kg body wt., daily via oral gavage for a period of 60 days. At the end of exposure period, rats were sacrificed; blood samples were collected and concentrations of cholesterol, triglycerides, glucose, insulin, adiponectin, reactive oxygen species (ROS) and oxidative damage to DNA were measured. Colon tissues were scored for tumors, and preserved in 10% formalin for observation of pathological changes. Rats that received WD + B(a)P registered increased levels of cholesterol,triglycerides and insulin in comparison to rats that received RD + B(a)P and also controls. Levels of glucose stayed the same between WD and RD for 25, and 50 µg B(a)P/kg body wt., but showed a significant difference (p < 0.001) at 100 µg B(a)P/kg body wt. with WD group showing high concentrations. The concentrations of adiponectin did not vary much between WD and RD groups for all the B(a)P doses used. Maximal levels of ROS and oxidative DNA damage in blood were noticed in WD + B(a)P rats compared to RD + B(a)P rats and controls. Additionally, the DNA damage was found to be B(a)P dose‐dependent. The colon tumor counts were more in B(a)P + WD rats compared to their B(a)P + RD counterparts, and also exhibited a B(a)P dose‐response relationship, with 100 µg B(a)P/kg registering greater counts. Adenomas with high grade dysplasia were prominent in B(a)P + WD rats compared to B(a)P + RD rats. Taken together, our findings revealed that WD potentiates the development of colon tumors induced by B(a)P through proinflammatory action, characterized by an increased generation of ROS and oxidative damage. Grant Funding Source : Supported by 5RO1CA142845‐04,5T32HL007735‐19,5R25GM059994‐11,Southern Regional Education Board