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Protein and gene expression of angiotensin II receptors (AT1 and AT2) in aorta of diabetic and hypertensive rats (1051.6)
Author(s) -
Romero Rodrigo,
ReséndizAlbor Aldo,
Rodriguez Jessica,
SanchezMuñoz Fausto,
Hong Enrique,
Villafaña Santiago
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.1051.6
Subject(s) - angiotensin ii receptor type 1 , diabetes mellitus , medicine , endocrinology , receptor , angiotensin ii , messenger rna , blood pressure , renin–angiotensin system , angiotensin receptor , aorta , streptozotocin , biology , gene , biochemistry
Diabetes and hypertension have been associated with cardiovascular disease and stroke. Some reports have been related the coexistence of hypertension and diabetes with increases in the risk of developing vascular complications. Recently some studies have shown contradictory results suggesting that in early stages of diabetes and hypertension exist a reduced functional response to vasoconstrictor agents like angiotensin II (Ang II) which plays an important role in blood pressure regulation mechanism through the activation of its AT1 and AT2 receptors. For that reason, the aim of this work was to study the gene and protein expression of receptors AT1 and AT2 in aorta of diabetic SHR and WKY rats. Diabetes was induced by the administration of streptozotocin (60 mg/kg i.p.). After 4 weeks of the onset of diabetes the protein expression was obtained by western blot and the mRNA expression by RT‐PCR. Our results showed that the hypertensive rats have a higher mRNA and protein expression of AT1receptors than normotensive rats while the AT2 expression was unchanged. On the other hand, the combination of diabetes and hypertension increased the mRNA and protein expression of AT1 and AT2 receptors significantly. In conclusion our results suggest that diabetes with hypertension modifies the mRNA and protein expression of AT2 receptors and these changes could be associated with the reduction in the response to Ang II in early stage of diabetes. Grant Funding Source : CONACYT CB‐2012‐01‐183660/COFAA