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In vivo demonstration of okadaic acid internalization in glutamatergic spinal motor neurons (1050.3)
Author(s) -
TorresVazquez Irma,
TorrechSantos Christian,
SerranoVelez Jose,
Rein Kathleen,
RosaMolinar Eduardo
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.1050.3
Subject(s) - okadaic acid , glutamatergic , neuroscience , internalization , excitatory postsynaptic potential , motor neuron , biology , chemistry , microbiology and biotechnology , phosphatase , spinal cord , phosphorylation , inhibitory postsynaptic potential , biochemistry , glutamate receptor , cell , receptor
The development of new techniques and reagents has facilitated the detailed study and characterization of endocytic pathways. We used okadaic acid (OA), a naturally occurring toxin produced by marine dinoflagellates of the genus Prorocentrum, that is a potent and selective inhibitor of the serine/theorine protein phosphatase, phosphoprotein phosphatase 1, combined with a minimally invasive method to retrogradely label neurons and their processes in an adult spinal motor circuit. We demonstrate the prevalent expression of connexin 35 gap junction proteins and plaques at dendrodendritic excitatory mixed synapses between spinal motor neurons and interneurons. In addition, we discovered that OA appears to be entering spinal motor neurons through a clathrin‐independent internalization mechanism that we are currently investigating. Grant Funding Source : Supported by MH‐086994; NSF‐10 62963, and NSF‐0964114