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The effect of CBD, CBG and a combination of CBD plus CBG on Haccat human skin carcinoma cells (1048.19)
Author(s) -
Javid Farideh,
Afshinjavid Saeed
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.1048.19
Subject(s) - chemistry , cytotoxicity , ic50 , cannabidiol , cytotoxic t cell , fetal bovine serum , cancer cell , mtt assay , pharmacology , cell culture , apoptosis , microbiology and biotechnology , biochemistry , in vitro , medicine , cancer , biology , cannabis , psychiatry , genetics
The effect of CBD, CBG and a combination of CBD plus CBG on Haccat human skin carcinoma cells Cannabinoid pharmacology has received attention in cancer research for their potential in combating cancer (Alexander et al., 2009). The present study investigated the potential anti‐tumour activity of cannabidiol and cannabigerol on skin tumour cells. Haccat cells (American Type Culture Collection) were grown and maintained in DBEM medium plus10% fetal bovine serum at 37 o C, 5% CO 2 .The cells were plated in 96‐well culture plates at a density of 1x10 4 cells/well and allowed to adhere at 37 oC for 24 hours. The following day, various doses of the compounds or were added to the cells and further incubated for 24, 48, 72 and 96 hours. Then the supernatant was removed and MTT (3‐(4,5‐dimethylthiazol‐2‐yl)‐2, 5‐diphenyltetrazolium bromide) was added for 4 hours. The ability of cells to form formazan crystals by active mitochondrial respiration was determined after dissolving the crystals in DMSO. Cytotoxicity was expressed as a relative percentage of the absorbance measured at 540 nm. CBD induced dose‐dependent cytotoxic effect (IC50 of 4.83+1.4, 4.75+0.06; 5.22+0.3 and 5.19+1.27 micromolar for 24, 48, 72 and 96 h contact time respectively). Addition of CBG also induced cell toxicity with IC50 of 1.96+0.3, 5.5+0.34, 8.32+0.19 and 7.85+1.47 for 24, 48, 72 and 96 h respectively. A combination of CBD plus CBG induced cell toxicity with an IC50 of 2.4 + 0.05 over 96 h contact time. The data suggest a significant (p<0.001) cytotoxicity when compounds are applied in combination. We thank GW Pharmaceuticals for providing cannabinoid extracts. Grant Funding Source : University of Huddersfield