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Examining the carcinogenic potential of pancreatic inflammation in obese mice with maspin staining (1047.10)
Author(s) -
Molteni Agostino,
Saeed Anwaar,
Glazyrin Alexey,
Pennington Vanessa,
Gawlik Mariah,
Saeed Ali,
Quinn Tim,
Herndon Betty
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.1047.10
Subject(s) - maspin , pancreas , inflammation , medicine , endocrinology , endocrine system , pancreatitis , pathology , cancer , metastasis , hormone
Chronic pancreatitis is associated with inflammation and adenocarcinoma. Unlike tumors in other sites, early pancreatic lesions upregulate maspin, a prognostic marker. This study evaluates pancreatic pathology in an OB/OB mouse model, to determine if inflammation leads to pre‐cancerous lesions that can be stained for maspin. A 10 animal control group and a group receiving TNFα receptor blocker (n=10) were evaluated. Pancreas collected at 2 months were stained by H&E and scored for inflammation and other damage. Anti‐maspin labeling was used to determine proliferative changes. H&E: Untreated: Ductal inflammation occurred only in exocrine pancreas but not endocrine. TNFα‐r block : less ductal inflammation occurred in exocrine pancreas but endocrine areas were similar. Anti maspin: Exocrine stain averaged 2.14 untreated vs 1.88 TNF‐α‐r block (p>0.05). Endocrine scores were 4.75 vs 7.43 on the same groups (p=0.03). No group differences occurred in serum insulin or blood glucose. Data suggest that pancreatic inflammation does not associate with anti maspin staining in the OB/OB model. Only exocrine pancreatic ducts were highly inflamed (H&E) and endocrine pancreas showed significant anti‐maspin staining. Further, maspin staining was unrelated to the systemic anti‐inflammatory treatment (TNFα‐r block). The relationship of maspin to other adipocytokines in the pancreas of this model remains an ongoing study