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Characterization of force sensing apparatus in human periodontal ligament fibroblasts (1046.10)
Author(s) -
Macarak Edward,
Braud Martin,
Wei Wenjie,
Giglou Kamyar,
Howard Pamela
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.1046.10
Subject(s) - periodontal fiber , blot , chemistry , gene expression , microbiology and biotechnology , laminin , immunohistochemistry , dystrophin , gene , biology , cell , biochemistry , dentistry , immunology , medicine
Characterization of force sensing apparatus in human periodontal ligament fibroblasts A putative force transfer complex (FSC) similar to that found in muscle tissue (dystrophin glycoprotein complex or DGC) has been characterized in human periodontal ligament fibroblasts (HPDLFs). Similar to the DGC, the FSC is a physical bridge that links the cell to the basement membrane (BM). HPDLFs were also subjected to compressive strain in vitro to mimic that used in orthodontic tooth movement and its effects on the gene expression of 14 genes evaluated. Methods: Using immunohistochemistry and Western blotting, HPDLFs were characterized with respect to either the presence or absence of DGC and BM components. To evaluate the effects of compressive force on HPDLF gene expression, mRNAs were quantified by RT‐ PCR. Results: DGC and BM proteins localized to HPDLFs by immunohistochemistry and further characterized in HPDLFs extracts by Western blotting; however, dystrophin was not identified. RT‐PCR revealed significant changes in gene expression of α‐sarcoglycan (p = 0.0009) and γ‐sarcoglycan (p < 0.0001), collagen III (p = 0.0191), and laminin (p = 0.0077) in HPDLFs that underwent cyclic compression. Conclusions: DGC and BM proteins, with the exception of dystrophin, are present in HPDLFs. Cyclic compressive forces caused significant changes in gene expression of some DGC and ECM components.