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Brassicaceae spp. spout extract inhibits ROS production induced by bisphenol A during the differentiation of 3T3‐L1 preadipocytes (1045.8)
Author(s) -
Lee Jong Seok,
Lee YoungJun,
Kim JaeHwan,
Kim DanBi,
Kim YoungHyun,
Lee OkHwan,
Lim JeongHo
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.1045.8
Subject(s) - brassicaceae , oxidative stress , antioxidant , superoxide dismutase , chemistry , reactive oxygen species , glutathione , sulforaphane , sod2 , biochemistry , glutathione reductase , biology , enzyme , botany , glutathione peroxidase
Bisphenol A (BPA), an environmental endocrine disrupting chemical, is contained in polycarbonate plastic products. BPA plays as a mixed agonist‐antagonist with respect to steroid hormones such as estrogens. Recent studies have suggested that BPA affects adipocytes diffenetiation and lipid accumulation by regulation of adipogenic transcription factors. In this study, we evaluated the influence of BPA on oxidative stress during the differentiation of 3T3‐L1 preadipocytes. We also evaluated that inhibitory effects of Brassicaceae spp. spout extracts such as Raphanus sativus L, Brassica oleracea var gongyloides, Brassica rapa var. glabra Regel on BPA‐induced oxidative stress during the differentiation of 3T3‐L1 preadipocytes. Our results showed that BPA treatment significantly increased ROS production in 3T3‐L1 cells compared with control by down‐regulating expression of antioxidant enzyme such as manganese superoxide dismutase (Mn‐SOD) and glutathione reductase (GR). However, Brassicaceae spp. spout extracts significantly inhibited BPA‐induced ROS production. In addition, Brassicaceae spp. spout extracts increased the level of the expression of antioxidant enzyme such as Mn‐SOD and GR. These results indicated that Brassicaceae spp. spout extracts consider as inhibitors that attenuate BPA‐induced adipocytes dysfunction.