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Clove extract regulates energy metabolism in differentiated C2C12 cells (1045.38)
Author(s) -
Tu Alex,
MossPierce Tijuana,
Jiang Alan,
Ford Paul
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.1045.38
Subject(s) - ampk , myogenesis , glycolysis , amp activated protein kinase , pyruvate carboxylase , coactivator , glucose uptake , protein kinase a , beta oxidation , nrf1 , downregulation and upregulation , biochemistry , metabolism , biology , chemistry , endocrinology , skeletal muscle , kinase , enzyme , insulin , mitochondrion , mitochondrial biogenesis , transcription factor , gene
An incidence of diabetes is rising dramatically worldwide. Plants and herbs have been used for the treatment of diabetes for centuries in folk medicine. Clove extract (CE) has been shown to respond similar to insulin by repressing genes encoding gluconeogenic enzymes; however, the mechanisms are essentially unknown. We investigated the effects of clove on metabolism in differentiated C2C12 myotubes and demonstrated that CE significantly increased glucose consumption as well as phosphorylation of AMP‐activated protein kinase (AMPK) and its substrate, acetyl‐CoA carboxylase (ACC). Additionally, CE transcriptionally regulated genes involved in metabolism were upregulated including SIRT1 (2‐fold) and PPARγ coactivator 1α (PGC1α) 2‐fold. Furthermore, treatment of CE dose‐dependently increased muscle glycolysis up to 23% and mitochondrial spare respiratory capacity up to 45%. Overall, our study suggested that CE potentially increases muscle glycolysis and fatty acid oxidation by activating the AMPK and SIRT1 pathways.