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Priming of the antiviral immune transcriptome by a mixed flavonoid‐fish oil supplement in adult overweight women (1045.23)
Author(s) -
CialdellaKam Lynn,
Nieman David,
Knab Amy,
Shanely R. Andrew,
Meaney Mary Pat,
Jin Fuxia,
Sha Wei,
Ghosh Sujoy
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.1045.23
Subject(s) - placebo , fish oil , immune system , quercetin , flavonoid , transcriptome , overweight , medicine , immunology , pharmacology , litopenaeus , endocrinology , physiology , biology , food science , gene expression , fish <actinopterygii> , biochemistry , obesity , gene , antioxidant , pathology , alternative medicine , fishery , shrimp
Flavonoids and fish oils have anti‐inflammatory and immune modulating influences. The purpose of this study was to determine if a mixed flavonoid‐nutrient‐fish oil supplement (Q‐Mix; 1000 mg quercetin, 400 mg isoquercetin, 120 mg EGCG, 220 mg EPA, 180 mg DHA) would alter genomic profiles in overweight, middle‐aged women. In this double‐blinded placebo‐controlled trial, women (n=48; age=56.1±8.3y; BMI=33.2±0.8kg/m2) were randomly assigned to either Q‐Mix supplement or placebo for 10 weeks. Whole blood derived mRNA, collected at 0‐ and 10‐wk, was analyzed using Affymetrix U133 Plus 2.0 microarrays. Plasma levels increased with Q‐mix supplementation by 388% for quercetin, 95% for EPA, 18% DHA, and 20% DPA. Gene Set Enrichment Analysis revealed upregulation of three pathways in Q‐Mix vs. placebo related to interferon‐induced antiviral mechanism. Consistent with this finding, over‐representation analysis also disclosed that infection, immune cell trafficking, and inflammatory pathways were inhibited in Q‐mix compared to placebo. Thus, relative to the placebo, a 10‐wk Q‐mix supplementation elicited a significant rise in plasma quercetin, EPA, DHA, and DPA and stimulated an antiviral whole‐blood transcriptomic response in adult overweight women.