Antioxidant and anti‐proliferative capacity of extractable phytochemicals from tropical fruits (pineapple, mango and papaya) (1045.18)

Fruits and vegetables (F&V) are universally promoted as healthy and prospective cohort studies support the evidence that their consumption is associated with a lower risk of all‐cause mortality and death from cardiovascular disease & cancer. In particular, tropical fruits such as Mango (MA, Mangifera indica cv. Ataulfo), papaya (PM, Carica papaya cv. Maradol) and pineapple (PE, Anana comosus cv. Esmeralda) have a vast array of phytochemicals with antioxidant and anti‐carcinogenic potential. The aim of this study was to compare the antioxidant profile and anti‐proliferative capacity of hydro‐methanolic (20:80 v/v) extracts obtained from freeze‐dried pulps of these fruits. MA had 2.3 & 2.7 more phenolic compounds (9.9±0.2 mgGAE/g DM) and 0.6 & 54.6 more ascorbic acid (956±2.2 mg/g DM) than PM or PE, respectively while PE had 0.2 & 2.8 more flavonoids (0.5±0.1 mgQE/g DM) than MA or PM. MA was more effective in scavenging DPPH and ABTS radicals (IC50 5.0 and 5.6 mg/mL) than PM (IC50 18.0 and 17.5 mg/mL) or PE (IC50 29.0 and 48.0 mg/mL). MA was more effective to inhibit the proliferation of RAW264.7 (mouse leukaemic monocyte/macrophage), L929 (mouse fibroblast) and HeLA (Cervix adenocarcinoma) cells at IC50 = 90, 138 and 187 mg/mL, respectively than PE (IC50 187, >200 and >200 mg/mL) or PM (IC50 >200 mg/mL) as assayed by the MTT Proliferation assay. However, all three fruit extracts were no effective (>200 mg/mL) to inhibit A549 Human lung adenocarcinoma cells. The specific phenolic profile and antioxidant capacity is related to the anti‐proliferative properties of these three tropical fruits.