Flavonoids and saponins extracted from black bean ( Phaseolus vulgaris L.) seed coats modulate lipid metabolism and biliary cholesterol secretion in C57BL/6 mice (1045.1)

Beans exert hypolipidemic activity, but this effect has not been attributed to any particular component, and the underlying mechanisms of action is unknown. The aim of this study was to extract, identify, and quantify the major saponins and flavonoids contained in black bean seed coats and to study their effects on lipid metabolism in rat primary hepatocytes and C57BL/6 mice. Phytochemical extraction was performed with 80 % methanol in water. The resulting extract was concentrated and lyophilized. Phytochemicals were identified by HPLC‐UV‐ELSD and HPLC‐MS‐TOF, the major compounds of the extract were flavonoids and saponins. In vitro and in vitro assays were performed to analyse the effects of major flavonoids and saponins contained in black bean seed coat on key proteins involved in lipid metabolism and biliary cholesterol secretion. An energy metabolism study was performed to determine whether the ingestion of these phytochemicals changed the proportion of lipids used as a substrate for energy generation. Serum biochemical parameters and lipids in the liver were measured and hepatic histological analysis was performed. Results suggested that flavonoids and saponins can prevent hepatic lipid accumulation by attenuating lipogenesis and stimulating fatty acid oxidation and cholesterol biliary excretion through the differentially regulation of LXR probably mediated by AMPK phosphorylationGrant Funding Source : Supported by the Nutrigenomic Research Chair Funds from Tecnologico de Monterrey‐Campus Monterrey