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Melibiose, a non‐digestible saccharide, promotes absorption of quercetin glycosides in rat small intestine with a novel mechanism (1044.9)
Author(s) -
Tanaka Seiya,
Shinoki Aki,
Hara Hiroshi
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.1044.9
Subject(s) - quercetin , aglycone , chemistry , melibiose , glycoside , rutin , glucoside , biochemistry , hydrolysis , antioxidant , stereochemistry , sucrose , maltose , medicine , alternative medicine , pathology
We examined effects of melibiose a non‐digestible saccharide, on intestinal absorption of quercetin glycosides, which are low bioavailability but have antioxidant, anti‐inflammatory and anti‐carcinogenic properties. We used water‐soluble quercetin glycosides (a mixture of ~7 glucose adducts of quercetin‐3‐ O ‐glucoside, Q3GM). Melibiose (150 µmol) with or without Q3GM (15 or 60 µmol) was directly injected into a ligated segment of rat jejunum in anesthetized rats or into the duodenum in conscious rats. Before and after an injection, the concentrations of quercetin derivatives in the portal blood collected through a cannula were measured by LC‐MS. Melibiose enhanced absorption of quercetin aglycon and total quercetin derivative including conjugated and methylated forms in situ experiments. We found a similar tendency in in vivo experiment using portal cannulated rats. Additionaly, we found that Q3GM was very rapidly hydrolyzed to Quercetin‐3‐ O ‐glucoside (Q3G) by a homogenate of the small intestinal mucosa, but melibiose promoted further hydrolysis of Q3G to quercetin aglycone. In conclusion, non‐digestible saccharides, melibiose, enhances intestinal absorption of quercetin glycosides with increasing hydrolysis to quercetin aglycone in the small intestinal lumen. This study suggests that melibiose contribute health promotive effects of quercetin glycosides.