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Urinary proanthocyanidin A2 as a biomarker of consumption of cranberry juice ő a pilot study (1044.3)
Author(s) -
Chen CY.,
Zampariello Carly,
Blumberg Jeffrey
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.1044.3
Subject(s) - urine , chemistry , cranberry juice , proanthocyanidin , biomarker , chromatography , ethyl acetate , morning , flavonoid , detection limit , food science , urinary system , polyphenol , medicine , biochemistry , antioxidant
The absence of a validated biomarker of cranberry intake has limited the ability to interpret results from many human studies, particularly long‐term clinical trials examining the effect of cranberries on health outcomes. Cranberries are rich in type A proanthocyanins, a flavonoid found in very few other foods. The objective of this study was to determine whether proanthocyanin dimer A2 (PAC‐A2) could be a useful biomarker of cranberry intake. Five healthy, premenopausal women were asked to consume 237 mL/d cranberry beverage containing 140 mg PAC, according to a weekly dosing schedule of 0 to 7 times daily over a 7 wk period. Eleven 24‐h and morning spot urine samples each were collected for PAC‐A2 analysis. Urine samples were treated with β‐glucuronidase and extracted with ethyl acetate. The ethyl acetate fraction was dried under N 2 gas, reconstituted in a NH 4 HCO 2 buffer, and extracted with an activated solid phase Bond Elut PBA cartridge. PAC‐A2 was separated by a Kinetex C18 column (4.6 x 150 mm x 2.6 µm) and quantified by LC‐MS/MS operating in negative ion mode. The variation in response of PAC‐A2 spiked to 11 urine samples collected from non‐study volunteers was 4.8%. The assay limit of quantification for urinary PAC‐A2 is 0.25 ng/mL. Results from this pilot study suggest that PAC‐A2 is not excreted in the urine in a frequency‐dependent manner corresponding with intake. Funded by the Cranberry Institute and USDA. Grant Funding Source : Supported by Cranberry Institute and USDA