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The bioavailability iron, zinc and copper in the rat due to their interactions (1042.8)
Author(s) -
Gordon Dennis,
Ellersick Mark
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.1042.8
Subject(s) - bioavailability , weanling , zinc , copper , chemistry , nutrient , factorial experiment , zoology , environmental chemistry , metallurgy , biology , materials science , endocrinology , pharmacology , mathematics , statistics , organic chemistry
There are many factors believed to influence nutrient bioavailability. However, possibly the most significant factors affecting the bioavailability of Fe, Zn and Cu, because of their similar chemical nature, are their levels in the body and dietary concentrations. The ultimate objective and results of this interaction study in rats and related studies in pigs and humans were used to formulate foods with optimum mineral concentrations to achieve optimum bioavailability. First determining the threshold levels of Fe, Zn and Cu needed to sustain adequate status in weanling rats, 36 groups of 5 animals per groups, were randomly arranged in a 3 (Fe) X 4(Zn) X 3(Cu) factorial. While all nutrients were constant in the AIN‐76 diet, dietary levels in ppm were: Fe, 18, 90 and 270; Zn, 8.5, 10.5, 42.5 and; Cu, 2, 10 and 40. Mineral status among all groups after 28 days ad libitum feeing was assessed by Hb levels, and liver and femur Fe, Zn and Cu concentrations. Significant interactions were observed for replication, Fe, Zn, Cu, Fe*Zn, Fe*Cu, Zn*Cu and Fe*Zn*Cu and with few exceptions all at P蠄0.01. The most significant and important interaction among Fe*Zn*Cu were/are illustrated using response surface methodology. Taking into consideration the most appropriate level of one element to be added to a food, estimates for the other two elements at that level could be determined and the process can be completed/repeated for all three elements. Grant Funding Source : Missouri Agricultural Experiment Station