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Ascorbate‐induced iron transport through ferroportin involves IRP2 and HIF2α (1042.2)
Author(s) -
Scheers Nathalie,
Sandberg AnnSofie
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.1042.2
Subject(s) - ferroportin , ferritin , iron status , dmt1 , western blot , chemistry , iron deficiency , biochemistry , iron homeostasis , medicine , transporter , metabolism , gene , anemia
In previous studies we have shown that ascorbate status of intestinal Caco‐2 cells affects the cellular levels of iron‐absorption related proteins (DMT1, DCYTB, ferritin, and ferroportin). The aim of the present study was to dig deeper into the ascorbate effects to investigate the mechanisms behind. We have measured ferroportin, IRP2, and HIF2α expression in response to different ascorbate concentrations with ELISA and Western blot analyses, which resulted in peaking protein levels at 100 µM followed by a decrease at higher ascorbate concentrations. In conclusion, Iron‐independent regulation of ferroportin by IRP2 seems to be responsible for the ascorbate‐mediated decrease in ferroportin with increasing ascorbate status. Grant Funding Source : The Swedish Council for Environment, Agricultural Sciences and Spatial Planning funded this study.