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Polymorphisms in the MGLL gene are associated with plasma LDL‐C response to a marine n‐3 PUFA supplementation (1038.1)
Author(s) -
Ouellette Catherine,
Cormier Hubert,
Rudkowska Iwona,
Lemieux Simone,
Couture Patrick,
Vohl MarieClaude
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.1038.1
Subject(s) - polyunsaturated fatty acid , single nucleotide polymorphism , genotype , biology , docosahexaenoic acid , medicine , endocrinology , genetics , fatty acid , zoology , gene , biochemistry
Omega‐3 (n‐3) polyunsaturated fatty acid (PUFA) supplementation from marine sources has an inconsistent effect on plasma low‐density lipoprotein cholesterol (LDL‐C) levels, as some individuals increase their levels while some others do not. Genetic factors may be involved in this inter‐individual variability observed in LDL‐C levels in response to an n‐3 PUFA supplementation. The objective of this study was to test whether SNPs within the MGLL gene, encoding the monoglyceride lipase, explain inter‐individual variability observed in plasma LDL‐C levels after an n‐3 PUFA supplementation. A total of 208 subjects consumed 5 g/d of a fish oil supplement, containing 1.9‐2.2g EPA and 1.1g DHA, for 6 weeks. Plasma lipids were measured before and after the supplementation. SNPs rs782440 and rs6776142 from MGLL were genotyped using the TaqMan technology (Life Technologies Inc. Burlington, ON, Canada). Results show that for rs782440 and rs6776142, there were differences in the mean variation in LDL‐C (delta LDL) between genotype groups (C/C, C/T and T/T) in an ANOVA adjusted for age, sex and BMI. Also, a chi‐square test showed differences in genotype frequencies for rs782440 (p<0.05) between responders and non‐responders defined on the basis of delta LDL, as well as a tendency for rs6776142 (p=0.099). In conclusion, two SNPs of the MGLL gene may influence the plasma LDL‐C variation in response to an n‐3 PUFA supplementation. Grant Funding Source : Supported by CIHR