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Microflora changes with an acute high fat diet (1037.13)
Author(s) -
Gonzalez Christopher,
Haar Lauren,
Engevik Melinda,
Jones W.
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.1037.13
Subject(s) - firmicutes , actinobacteria , gut flora , bacteroidetes , biology , lipopolysaccharide , cytokine , proteobacteria , cardioprotection , intestinal permeability , immune system , bacteria , endocrinology , medicine , immunology , 16s ribosomal rna , genetics , ischemia
Activation of NF‐κB signaling and ensuing protective gene transcription in the heart is the basis for cardioprotection in the murine heart following an acute (24h) high fat diet (HFD). This protection is strongest after 2wks of high fat feeding and declines thereafter. The exact connection between acute high fat feeding and NF‐κB activation is not yet fully understood. Long‐term fat absorption in the intestine is associated with increased vascular permeability, decreased numbers of gram‐positive bacteria, and cytokine release into lymphatic circulation. We considered the novel hypothesis that an acute HFD affects the intestinal microflora. Bacterial DNA from stool samples, luminal flushes, and mucosal scrapings were isolated from 24h high fat fed mice and changes in the phyla Firmicutes, Actinobacteria, Bacteroidetes, and Y‐Proteobacteria measured by quantitative polymerase chain reaction. Our data indicates a significant increase in Actinobacteria in stool post high fat feeding. No statistically significant differences were found among bacteria in the flushes or mucosa at the 24hr time point. Conceivably, activation of pattern recognition receptors, or increased release of immune modifiers such as cytokines, known activators of NF‐κB systemically, could result from the observed changes in GI microflora post acute HFD. Our pilot data suggests focusing on cytokine regulation in response to short term high fat feeding. Further experimentation to identify the changing strains of Actinobacteria and quantify levels/activation of TRL4 and IL‐6 post 24h HFD, as well as experiments to directly link the changes in flora to cardioprotection are underway.