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Butyrate‐induced upregulation of intestinal glucose transport represents a possible nutrient therapy for individuals with malabsorptive disorders (1035.14)
Author(s) -
Mangian Heather,
Mangian Heather,
Tappenden Kelly
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.1035.14
Subject(s) - butyrate , glut2 , proglucagon , cecum , medicine , ileum , jejunum , endocrinology , saline , small intestine , glucose transporter , cannula , chemistry , biology , glucagon like peptide 1 , biochemistry , insulin , diabetes mellitus , surgery , fermentation , type 2 diabetes
Individuals with significant intestinal malabsorption face challenges digesting and absorbing sufficient nutrients. Therapies that increase intestinal nutrient processing capacity are needed. Objective: Our objective was to identify the impact of butyrate (B) upon intestinal glucose transport capacity. We hypothesized that ileal B would increase jejunal glucose transport via increased glucose transporter 2 (GLUT2) abundance. Further, mechanistic stimulation of enteroendocrine L cells by B was examined. Method: Sprague Dawley rats were surgically modified with an ileal cannula for infusion of either 10 mM B or saline solution (for 96 or 156m) and a portal cannula for blood sampling. All rats received the tracer (3‐O‐[methyl‐14C] ‐D‐glucose (8 uCi), but were randomized to be fed (3 mg/kg BW D‐glucose) or fasted (0 mg/kg BW D‐glucose). Results: Glucose absorption decreased after extended ileal saline infusion compared to a shorter saline infusion (AUC 4,640,245 vs. 2,567,799 DPM/ml2, P< 0.05) . B abolished this decrease. B increased jejunal GLUT2 (by 23% ; P<0.05) but not SGLT1 mRNA. Proglucagon mRNA abundance was 2‐fold higher in the cecum of B rats (B 0.679 vs. S 0.332, proglucagon mRNA/18S; P<0.0014). B also increased Free Fatty Acid Receptor 2&3 (FFAR2 and FFAR3) mRNA abundance in the cecum. Conclusions: Treatment of the distal intestine with B increases the glucose absorptive capacity of the proximal intestine. Grant Funding Source : NIDDK 1 R01 DK57682