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Novel aerobic bacteria: isoflvones‐biotransformation of Pueraria radix extract (1034.8)
Author(s) -
Kim Inhye,
Kwon JungEun,
Koo Hyun Jung,
Park TaeHo,
Sohn EunHwa,
Jang SeonA,
Kim HaengRan,
Kang Se Chan
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.1034.8
Subject(s) - equol , daidzin , puerarin , daidzein , pueraria , isoflavones , lobata , chemistry , food science , bacteria , traditional medicine , biology , biochemistry , medicine , endocrinology , genistein , genetics , alternative medicine , pathology
Background: Daidzen and puerarin are the most common dietary isoflavones in Pueraria radix. Equol is an isoflavandiol metabolized from daidzin and puerarin by bacterial flora in the intestines. The metabolites of isoflavones play a key role in the availability of these compounds in the intestine. Aim: This study was therefore to screen human intestinally derived bacteria for the ability to produce equol or its derivatives from daidzein in the root of P. lobata (Wild.) Ohwi. Methods: To compare the conversion abilities of selected bacteria by different concentrations of P. lobata , we cultured the bacteria for 24 h in the GAM supplemented to 2.0% P. lobata . Results: We selected a strictly aerobic bacterium from candidate which converted daidzin to equol as well as puerarin to equol. The isolate was able to convert the isoflavones, daidzein to equol, equol precursors and derivates. Conclusions: This study was conducted with producing equol using P. lobata , good sources that an abundant precursor of equol, fermented by the newly selected microbes. This study was carried out with the support of cooperative research program for Agricultural Science & Technology Development (Project No. PJ008398022013), Rural Development Administration, Republic of Korea. Grant Funding Source : This study was supported by cooperative research program for RDA (PJ008398022013)