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Effect of commercial sweetener intake on the NF‐κB and AP‐1 pro‐inflammatory pathways in liver and small intestine in mice (1034.10)
Author(s) -
EspinoBarrosFlores Adamaris,
Estrada José,
PliegoRivero Bernardo,
Contreras Irazu
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.1034.10
Subject(s) - sucralose , ingestion , population , small intestine , medicine , immune system , endocrinology , chemistry , food science , immunology , environmental health
Commercial sweetener intake has increased over the last few years, not only in patients diagnosed with a chronic non‐communicable disease, but in the general population. These sweeteners are deemed safe for consumption; however, the effect of their constant use on the immune system is unknown. Objectives: To determine the effect of frequent sweetener intake on NF‐κB and AP‐1 signaling pathways in the liver and intestine of BALB/c mice. Methods: 14 week old BALB/c mice (male and female) were grouped (6 mice/per group, male and female) as follows: 1) control (water); 2) sucrose (10%); 3) sucralose (1%) and 4) rebaudioside A (1%). Sweeteners were administered orally in drinking water for 6 weeks. Food and liquid intake was measured daily and weight was measured every week, from week 8 to week 14. After treatment, mice were sacrificed to obtain the proteins from liver and small intestine. Analysis of total, as well as phosphorylated, protein expression for IκBα, c‐Jun, JNK and ERK was performed by WB. Results: Our results show that sweetener intake does not appear to have an impact on weight; however, we observed alterations in water and food consumption. Liquid intake was increased in the sucrose groups, whereas food ingestion was higher in the sucralose group (p蠄0.05). Expression of total proteins was not altered, compared to the control group; nonetheless, we still have to determine if there are alterations in the expression of the phosphorylated proteins.