Premium
Heat stress during differentiation of porcine adipocytes is accompanied by increased expression of adipocyte marker genes and downregulation of inflammatory genes (1033.2)
Author(s) -
Lu Hang,
Wang Henxiao,
Ajuwon Kolapo
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.1033.2
Subject(s) - adipocyte , adipose tissue , leptin , adiponectin , medicine , endocrinology , downregulation and upregulation , gene expression , biology , gene , chemistry , andrology , biochemistry , insulin , obesity , insulin resistance
Global increase in ambient temperature as a result of climate change imposes heat stress on both humans and livestock. For pigs, an important food animal and a biomedical model, the implication of sustained heat stress on growth, physiology and metabolism of the animal is still unclear. Because of the role that adipose tissue plays in the regulation of overall metabolism and growth efficiency in the pig, this study was conducted with the objective of determining the impact of heat stress during adipocyte differentiation on expression of mature adipocyte and inflammatory marker genes. Preadipocytes isolated from the subcutaneous adipose depot were differentiated under normal (37 o C) or heat stress (41.5 o C) condition for 3, 6 or 9 days. Gene expression was determined by RT‐PCR. Fold expression of genes at 41.5 o C was determined relative to 37 o C after normalization with 18s. Heat stress induced 1.5 and 1.3 fold increase in the expression of PPARγ on days 3 and 6, with no change on day 9. Expression of CEBPα was induced by heat stress by 2.4, 2.6 and 2.2 fold on days 3, 6 and 9 respectively. Expression of adiponectin (3.5, 1.9 and 3.3 fold), leptin (2.0, 2.7 and 2.3 fold), HSP70 (2.9, 3.5 and 2.2 fold), HSP90 (2.7, 5.2 and 4.5 fold) was higher in heat stressed adipocytes on days 3, 6 and 9 respectively. However, heat stress led to a reduction in the expression of IL‐6 (0.3, 0.3 and 0.2 fold) and TNFα (0.3, 0.5 and 0.1 fold) on days 3, 6 and 9 respectively, relative to cells differentiated at 37 o C. Therefore, heat stress leads to significant alteration in adipocyte metabolism in a way that may be consequential for the health and growth performance of pigs.