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Butyrate increased lifespan in C. elegans (1025.13)
Author(s) -
Gao Chenfei,
Cao Tuan,
Martin Roy,
Keenan Michael,
Greenway Frank,
Finley John,
Burton Jeff,
Johnson William,
Enright Frederick,
Zheng Jolene
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.1025.13
Subject(s) - sodium butyrate , butyrate , caenorhabditis elegans , propionate , sodium propionate , fermentation , tributyrin , chemistry , food science , starch , biology , biochemistry , gene , lipase , enzyme
Obesity is a growing public health dilemma around the world. Resistant starch (RS), fermented resistant starch, and short‐chain fatty acids (SCFAs) reduce intestinal fat deposition (IFD) in wild type Caenorhabditis elegans (N2, C. elegans ), and increase the healthspan marker, pharyngeal pumping rate (PPR). We hypothesized that the fermentation products, SCFAs, would improve lifespan in C. elegans model. N2 was maintained in liquid culture media supplied with 5‐fluoro‐2’‐deoxyuridine (FUdR, 0.24mM). Control group was fed with E. coli OP50 only. Experimental group received additional sodium butyrate (0.3mM, 1mM), sodium acetate trihydrate (0.3mM), sodium propionate (100mM), and tributyrin (3mM). Butyrate (0.3mM) increased mean survival time during 35 day study (P=0.03) in the C. elegans . Other treatments mildly sustained the lifespan of C. elegans . These data proved that the SCFA butyrate extended lifespan in addition to creating a negative energy balance in C. elegans model.