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Potential of glycated proteins produced during aging of cheddar cheese to modulate fecal bacteria from obese mice ex vivo and protect against colon inflammation (1018.5)
Author(s) -
Yuan Jialing,
Noratto Katherine,
Munske Gerhard,
Pilla Paulina,
Mohanty Indira,
Alcantara Zapata Diana,
Noratto Giuliana
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.1018.5
Subject(s) - feces , bacteria , fermentation , ex vivo , food science , chemistry , lactobacillus , gut flora , firmicutes , casein , digestion (alchemy) , bifidobacterium , microbiology and biotechnology , biochemistry , biology , in vitro , chromatography , genetics , 16s ribosomal rna
Cheddar cheese aging promotes the production of glycated proteins with potential to improve intestinal health. We investigated major changes on proteins of aged cheddar cheese (Cougar Gold) during ripening up to 24 months using HPLC/MS‐MS. The proteins effects on fecal bacteria from obese mice after in vitro intestinal digestion, and the effects of metabolites produced during fecal fermentation were evaluated. We further assessed the effects of metabolites from fecal fermentation on production of reactive oxygen species (ROS) in colon epithelial HT‐29 and the fibroblast CCD‐18Co cells challenged with E. Coli lipopolysaccharides (LPS) following standard analytical procedures. Results demonstrated that cheese aging (6‐24 months) produced soluble glycosylated proteins of MW ~ 23 kDa mainly derived from α‐S1‐ and β‐casein. Cheese glycated proteins were resistant to in vitro gastrointestinal digestion and changed the relative abundances of fecal bacteria by increasing Bacteroidetes/Firmicutes ratio and Bifidobacterium compared to the casein fermented controls. These changes resembled relative abundances of bacteria in feces from lean mice control. Furthermore, metabolites produced during fecal fermentation protected both the HT‐29 and CCD‐18Co cells from LPS‐induced ROS. We are currently investigating the molecular targets of glycated proteins and metabolites produced after fecal fermentation that protect colon cells from inflammation. These results are relevant for gut health.