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A comparison of bacterial communities in feces of breastfed infants determined by 454 pyrosequencing and Illumina MiSeq (1017.5)
Author(s) -
Mosely Janae,
Williams Janet,
Yahvah Katherine,
Riley Mara,
Settles Matt,
Brooker Sarah,
Lackey Kimberly,
McGuire Mark,
McGuire Michelle
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.1017.5
Subject(s) - pyrosequencing , feces , biology , species evenness , metagenomics , illumina dye sequencing , genetics , dna sequencing , microbiology and biotechnology , ecology , species diversity , gene
Because various molecular techniques are used to evaluate microbial community structure in biological samples, understanding if results are comparable among methods is critical. We hypothesized two of the most commonly‐used techniques, 454 pyrosequencing and Illumina MiSeq, similarly describe bacterial communities in breastfed infants’ feces. To test this, DNA from 25 samples was processed using both techniques; universal primers designed to amplify the V1‐V3 region of the 16S rRNA bacterial gene were used. Bacterial community profiles were clustered using Bray‐Curtis dissimilarity and the average linkage algorithm. Of the 15 most abundant genera detected in the samples, only 8 were common between the 2 methods. Diversity was similar (Shannon, P=0.08), but evenness was different between methods (Simpson, P=0.001). Our data somewhat support the hypothesis that these methods produce similar results in terms of diversity; however, the methods differ in terms of overall community composition and evenness. In conclusion, these potential differences need to be considered when comparing bacterial community data across sequencing methods ‐ at least for feces of breastfed infants.