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NAADP evokes calcium signals and plays a critical role in downstream physiological responses in naïve T cells (1013.5)
Author(s) -
Ali Ramadan,
Camick Christina,
Wiles Katherine,
Slama James,
Giovannucci David,
Wall Katherine
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.1013.5
Subject(s) - jurkat cells , ryanodine receptor , second messenger system , microbiology and biotechnology , calcium signaling , endoplasmic reticulum , stimulation , receptor , chemistry , signal transduction , biology , t cell , biochemistry , immunology , immune system , endocrinology
Nicotinic acid adenine dinucleotide phosphate (NAADP) is the most potent Ca 2+ mobilizing second messenger discovered to date and has been implicated in Ca 2+ signaling in human Jurkat lymphoma T cells via ryanodine receptor activation. In contrast the role of NAADP in Ca 2+ signaling or the identity of the Ca 2+ stores targeted by NAADP in conventional naïve T cells has not been investigated. In the current study, we demonstrated for the first time to our knowledge the importance of NAADP in the generation of Ca 2+ signals in murine conventional naïve T cells. Combining live‐cell imaging methods and a pharmacological approach using the NAADP antagonist Ned‐19, we addressed the impact of NAADP on the generation of Ca 2+ signals evoked by TCR stimulation and the role of this signal in downstream physiological endpoints such as proliferation, cytokine production, and other responses to stimulation of murine naïve T cells. Moreover, we demonstrated that acidic stores in addition to the endoplasmic reticulum are the relevant Ca 2+ stores that were sensitive to NAADP in naïve T cells. Grant Funding Source : Supported by NIH GM100444