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Magnolol affects the expression of polyamine biosynthesis and catabolism‐linked proteins in PC3 human prostate cancer cells in vitro (1011.1)
Author(s) -
McKeown Brendan,
Hurta Robert
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.1011.1
Subject(s) - polyamine , magnolol , ornithine decarboxylase , ornithine decarboxylase antizyme , protein kinase b , pi3k/akt/mtor pathway , chemistry , spermine , biology , microbiology and biotechnology , biochemistry , endocrinology , signal transduction , pharmacology , enzyme
This study examined the effect of magnolol on the expression of proteins involved in polyamine biosynthesis/catabolism in PC3 prostate cancer cells in vitro . Magnolol, 80 μM for 6 hours, decreased cellular viability by ~50%. Magnolol exposure decreased expression of ornithine decarboxylase (ODC), a key protein in polyamine biosynthesis, and antizyme‐inhibitor (AZI) with no apparent change to expression of antizyme (AZ), an ODC inhibitor. Expression of spermidine/spermine N1‐acetyltransferase, a key protein in polyamine catabolism, was also decreased. These magnolol‐mediated changes to ODC & AZI expression suggest that changes in polyamine biosynthesis would occur. An AZI/AZ ratio decrease in response to magnolol favors growth repression of PC3 cells. Cellular signaling proteins were also evaluated, with no apparent change occurring in ERK1, ERK2, p‐ERK1, p‐ERK2, p38, p‐JNK2, PI3Kp110 & AKT expression. Decreased expression of p‐p38, JNK1, JNK2, PI3Kp85, p‐PI3Kp85 & p‐AKT and increased expression of p‐JNK1 were observed. This study demonstrates that magnolol can alter the behavior of human prostate cancer cells in vitro by affecting activities associated with growth regulation. (Supported by the Jeanne and J.‐Louis Levesque Foundation.) Grant Funding Source : Supported by the Jeanne and J.‐Louis Levesque Foundation