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Effects of exposure to bisphenol A on hepatocytes and pre‐adipocytes in vitro (1010.7)
Author(s) -
Macis Elias,
Kostrominova Tatiana
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.1010.7
Subject(s) - dna fragmentation , apoptosis , trypan blue , viability assay , bisphenol a , chemistry , in vitro , fragmentation (computing) , adipogenesis , andrology , microbiology and biotechnology , biology , biochemistry , programmed cell death , medicine , ecology , organic chemistry , epoxy
In recent years, environmental pollutants/endocrine disrupting chemicals (EDCs) have received special attention as metabolic disruptors that produce adverse developmental, reproductive, neurological, cardiovascular, metabolic and immune effects. Bisphenol A (BPA) is one of the most abundant EDCs present in the environment. We focused our study on the effects produced by the exposure to BPA on cell viability, apoptosis and DNA fragmentation in FaO hepatocytes and 3T3 pre‐adipocytes in vitro. In particular, we were interested whether there are considerable differences in the sensitivity to cytotoxic effects of BPA in hepatocytes and pre‐adipocytes when evaluated under the same experimental conditions. Exposure to 10 and 100 μM of BPA showed that although morphology of both cell lines was affected after 24 and 48 hours of exposure, the effect on 3T3 pre‐adipocytes was more pronounced. Most probably exposure to BPA attenuated cell growth or caused cell detachment since the number of viable cells attached to the substrate did not show statistically significant differences when evaluated by trypan blue exclusion method. Several methods for apoptotic DNA fragmentation evaluation were compared. Qiagen DNeasy kit for DNA isolation showed the best detection sensitivity. Exposure to 100 μM of BPA for 48 hours showed presence of apoptotic DNA ladder when evaluated by gel electrophoresis. Support: IUN and IUSM‐NW. Grant Funding Source : Undergraduate Research Fund IUN