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Tet1/3 mediated epigenetic regulations in male germ cell development
Author(s) -
Luk Alfred CS,
Ng Shuk Hang,
Tao Jiajie,
Chan WaiYee,
Lee TinLap
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.lb92
Subject(s) - epigenetics , somatic cell , biology , germ cell , embryonic stem cell , dna methylation , stem cell , microbiology and biotechnology , 5 hydroxymethylcytosine , genetics , gene expression , gene
Previous epigenetic studies focused mainly on 5‐methylcytosine (5mC), emerging evidence suggests that 5‐hydroxymethylated cytosine (5hmc) could be another functional epigenetic modification in eukaryotic genome as demonstrated during differentiation of neurons and embryonic stem cells. However, its roles in male germ cell development remain unexplored. Our preliminary data demonstrated decreasing expression of ten‐eleven translocation (Tet) protein family after serial passages of C18–4 cells (mouse type A spermatogonia), suggesting potential correlation on epigenetics and aging. Moreover, we showed a higher expression level of Tet1/3 in stem‐cell like C18–4 cells than in somatic TM4 (mouse Sertoli) cell. Our dot‐blot results also demonstrated a higher genomic 5hmC content in C18–4 than in TM4 cells, implying possible stemness‐related regulation of 5hmC. Taken together, these data revealed a potential regulation of 5hmC/Tet in male germ cell development, which furthered our knowledge on male reproductive health and infertility control.